Ose membrane (0.45 mm pore size, Biorad) and left to dry for at least 30 minutes at room temperature. The spotted membranes were washed three times with PBST (0.05 Tween 20 in phosphate-buffered saline or PBS pH 7.4) applying a constant vacuum flow using SNAP i.d. Protein Detection System (Millipore) and blocked for 1 h at roomNeutralizing Epitope Guiding Bacterial Clearancetemperature in PBST bu